Histochemical changes in host tissues from Plasmodium cynomolgi B infected rhesus monkey (Macaca mulatta).

ABSTRACT

Plasmodium cynomolgi B has been used to infect the rhesus monkey to study the histochemical changes (lipid infiltration, glycogen, protein, DNA and RNA) in liver, kidney and spleen during early (exoerythrocytic) and late (chronic) stages of malarial infection. Infected liver showed significant lipid infiltration during exoerythrocytic and erythrocytic (acute phase) stage of infection. Kidney showed lipid deposition during acute phase of infection while spleen sections were negative for lipid depositions. As a result of malarial infection there was significant depletion of glycogen in liver during exoerythrocytic stage of infection. Glycogen content increased in liver and kidney during erythrocytic stage of infection. The spleen which is the main target of immunopathology in malaria showed no change in glycogen content. During exoerythrocytic phase host tissue organs showed no change in protein and nucleic acids while erythrocytic phase showed slightly increased proteins in liver and kidney. Nucleic acids became decreased in liver and spleen during erythrocytic phase of infection. The parasite used in this study has a defined prepatent period, can be cyclically passaged with ease and non fatal in nature.