Cell population growth in chick blastoderms cultured in vitro.
Indian J Exp Biol
;
1990 Jun; 28(6): 526-30
Article
in English
| IMSEAR
| ID: sea-61957
ABSTRACT
Primitive streak stage chick blastoderms were cultured in vitro up to 30 hr by New's technique. Chick blastoderms reaching stages 4 to 12 in vitro cultures and in ovo were harvested and homogenized to release cell nuclei. Fluorescent ethidium bromide-stained nuclei in homogenates were counted in Neubauer's chamber and the size of total blastoderm cell population was determined. Linear regression analysis revealed that both in ovo and in vitro chick blastoderm cell population grows in a biphasic manner with comparable cell population doubling times and the morphogenesis is not affected in vitro during the culture period.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Blastoderm
/
Cell Count
/
Cell Division
/
Cells, Cultured
/
Chick Embryo
/
Regression Analysis
/
Animals
/
Microscopy, Fluorescence
Type of study:
Diagnostic study
Language:
English
Journal:
Indian J Exp Biol
Year:
1990
Type:
Article
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