Cytosine methylation inhibits expression of hsp-CAT gene in Drosophila cells.
Indian J Exp Biol
;
1997 Mar; 35(3): 219-21
Article
in English
| IMSEAR
| ID: sea-62014
ABSTRACT
Chloramphenicol acetyl transferase gene under control of hsp promoter (hsp-CAT gene) was introduced and expressed upon heat shock in Drosophila cells at 48 and 72 hr following transfection. Expression of CAT gene was remarkably reduced when DNA methylated at CpG sites was used although presence of methylated plasmid DNA could be demonstrated in cells at 48 and 72 hr. Thus, in Drosophila cells exogenously introduced methylated DNA is expressed differently from unmethylated DNA.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Chloramphenicol O-Acetyltransferase
/
Gene Expression Regulation, Enzymologic
/
Promoter Regions, Genetic
/
Genes, Insect
/
DNA Methylation
/
Cytosine
/
Drosophila melanogaster
/
Animals
Language:
English
Journal:
Indian J Exp Biol
Year:
1997
Type:
Article
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