Reduction of vitrification in in vitro raised shoots of Chlorophytum borivilianum Sant. & Fernand., a rare potent medicinal herb.
Indian J Exp Biol
;
2006 Jun; 44(6): 499-505
Article
in English
| IMSEAR
| ID: sea-62090
ABSTRACT
Reduction of vitrification in in vitro raised shoots derived from shoot bases and immature floral buds along with inflorescence axis used as explants of C. borivilianum, a rare medicinal herb is described. Shoot multiplication was obtained on MS medium with 2 mg l(-1) benzylaminopurine (BAP) + 0.1 mg l(-1) indole-3-butyric acid (IBA) and MS medium with 2 mg l(-1) kinetin (Kin) + 0.1 mg l(-1) 2,4-dichlorophenoxy acetic acid (2,4-D) from shoot bases and inflorescence axis respectively. Best multiplication rates were obtained from both the explants on MS medium with 2 mg l(-1) BAP. Vitrification of shoots in cultures appeared during the multiplication stage. Culture bottles with aerated caps reduced the vitrification to 80%. Reduction of BAP concentration from 2 mg l(-1) to zero during subsequent subcultures also minimized vitrification. Use of 0.5-2 mg l(-1) Kin produced healthy shoots when compared to BAP. In vitro raised shoots rooted on Knop salts containing iron and vitamins of MS medium, 2 mg l(-1) IBA and 0.1% activated charcoal. About 80% plantlets survived upon soil transfer. Scanning electron microscopic and image analyzer studies reveal the morphological structural differences between the leaves of normal and vitrified plantlets.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Plants, Medicinal
/
Purines
/
Time Factors
/
Benzyl Compounds
/
Image Processing, Computer-Assisted
/
Microscopy, Electron, Scanning
/
Plant Shoots
/
Plant Roots
/
Cytokinins
/
Herbal Medicine
Language:
English
Journal:
Indian J Exp Biol
Year:
2006
Type:
Article
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