Your browser doesn't support javascript.
loading
Effect of cholesterol and 7-beta hydroxycholesterol on glutathione status and nitric oxide production in murine peritoneal macrophages.
Indian J Exp Biol ; 2005 Jun; 43(6): 503-8
Article in English | IMSEAR | ID: sea-62329
ABSTRACT
Present study was conducted to observe the effect of cholesterol and oxidized cholesterol (7beta-hydroxycholesterol,7beta-OH) on the nitric oxide (NO) production and the redox ratio by lipopolysaccharide-stimulated macrophages. Dose-dependent decrease in NO levels was seen with both cholesterol and 7beta-OH at different incubation intervals (6,12,18,24 hr) and concentrations (2.5,5,7.5microg/ml). On comparison, a significant decrease in the NO was observed at 24 hr interval in 7beta-OH exposed cells with all respective concentrations of cholesterol. Incubation with 7beta-OH also resulted in significant increase in levels of oxidized glutathione (GSSG) and decrease in reduced glutathione (GSH), while cholesterol showed no effect on GSSG levels. Moreover, GSH levels were lowered only at highest concentration (7.5microg/ml), and at longer incubation intervals (18,24 hr) with cholesterol exposure. This altered the redox status in both cholesterol/7beta-OH treated macrophages. Increased redox ratio and decreased NO levels indicated increased oxidative stress and decreased vasodilation by 7beta-OH compared to cholesterol.
Subject(s)
Full text: Available Index: IMSEAR (South-East Asia) Main subject: Oxidation-Reduction / Oxygen / Time Factors / Female / Lipopolysaccharides / Cholesterol / Macrophages, Peritoneal / Oxidative Stress / Nitric Oxide Synthase / Dose-Response Relationship, Drug Language: English Journal: Indian J Exp Biol Year: 2005 Type: Article
Full text: Available Index: IMSEAR (South-East Asia) Main subject: Oxidation-Reduction / Oxygen / Time Factors / Female / Lipopolysaccharides / Cholesterol / Macrophages, Peritoneal / Oxidative Stress / Nitric Oxide Synthase / Dose-Response Relationship, Drug Language: English Journal: Indian J Exp Biol Year: 2005 Type: Article