An enzyme linked immunosorbent assay for monitoring Bacillus sphaericus toxin.
Indian J Exp Biol
;
1991 Oct; 29(10): 953-7
Article
in English
| IMSEAR
| ID: sea-62466
ABSTRACT
Larvicidal proteins of B. sphaericus H5a5b (strain VCRC B42), purified by ion-exchange chromatography were used to raise antibodies in rabbits. The antibodies were specific in reacting to alkali-solubilized fractions from whole cells of toxic strains only. Ouchterlony immunodiffusion showed homology in toxin structure between strains of different serotype. A sandwich ELISA using avidin-biotin amplification was standardized. The lowest detectable limit was 6.25 ng/ml. Near linear quantitative binding of the antigen was found in the range 25-200 ng/ml. The growth, toxin level and LC50 values during various stages of fermentation of B. sphaericus strains 1593 and B42 were compared. There was significant correlation between LC50 values and toxin levels as measured by ELISA.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Bacillus
/
Bacterial Toxins
/
Enzyme-Linked Immunosorbent Assay
/
Blotting, Western
/
Chromatography, Ion Exchange
/
Immunodiffusion
/
Fermentation
Language:
English
Journal:
Indian J Exp Biol
Year:
1991
Type:
Article
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