Monte Carlo method for bioluminescence tomography.

ABSTRACT

Bioluminescence imaging plays an important role in the areas of cancer biology, cell biology, gene therapy, and so on. The 2D planar bioluminescent imaging has been transformed into a 3D framework by bioluminescence tomography (BLT) that enables bioluminescent source reconstruction in a mouse using a modality fusion approach. To solve this BLT problem, a geometrical model of the mouse is usually built from a CT/micro-CT/micro-MRI scan, which facilitates the assignment of optical parameters to various anatomical regions in the model. This optical model is then used to facilitate BLT. The forward model is based on Monte Carlo simulation to calculate the diffuse light flux on the surface of the mouse. The forward model data are used to define the imaging system and perform the BLT reconstruction. In this paper, we report the reconstruction of sources inside a heterogeneous highly scattering physical phantom to demonstrate the feasibility of this Monte Carlo based BLT method.