Significance of regional difference in ion concentrations in lizard, Hemidactylus flaviviridis (Rüppell): assessment of ionic influence on sperm motility in vitro.
Indian J Exp Biol
;
2003 Dec; 41(12): 1431-5
Article
in English
| IMSEAR
| ID: sea-62646
ABSTRACT
A gradual increase in the concentration of Ca2+ from anterior to the posterior region was observed when mono- and divalent cations were estimated in different segments of the epididymis in wall lizard. Na+ and K+ levels increased from anterior to middle segment but declined significantly in the posterior segment. However, no significant difference in the levels of Mg2+ was observed in various segments. To study the influence of mono- and divalent cations on sperm motility in vitro, the spermatozoa from posterior region of the epididymis were incubated in medium with varying concentrations of Na+, K+, Ca2+ and Mg2+. Spermatozoa were non-motile when suspended in Na+-free medium. Addition of NaCl induced the acquisition of sperm motility in a concentration-dependent manner. Further, amiloride, a Na+-influx blocker, markedly reduced the Na+-induced forward progressive motility. Unlike Na+, the presence of K+ or Ca2+ in the incubation medium reduced the motility of spermatozoa even at very low concentrations. The inhibitory effect of Ca2+ decreased when nifedipine, a Ca2+-influx blocker, was added to the medium. Mg2+ at high concentrations only was able to reduce the forward progressive motility.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Osmolar Concentration
/
Sperm Motility
/
Male
/
Cations
/
Lizards
/
Animals
Language:
English
Journal:
Indian J Exp Biol
Year:
2003
Type:
Article
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