Site specific integration of FLP recombinase in BHK-21 cell line.
Indian J Exp Biol
;
2003 Jun; 41(6): 570-3
Article
in English
| IMSEAR
| ID: sea-62740
ABSTRACT
A binary system for gene activation and site specific integration based on conditional recombination of transfected sequences mediated by FLP recombinase from yeast was implemented in mammalian cells. In several cell lines, FLP rapidly and precisely recombined copies of its specific target sequences to activate an otherwise silent beta-galactosidase reporter gene. Clones of marked cells were generated by excisional recombination within a chromosomally integrated copy of the silent reporters. These clones exhibited intense blue colour with X-Gal staining solution.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
DNA
/
Fungal Proteins
/
Base Sequence
/
Cell Line
/
Cricetinae
/
DNA Nucleotidyltransferases
/
Animals
Language:
English
Journal:
Indian J Exp Biol
Year:
2003
Type:
Article
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