Differential acetylcholinesterase activity in rat cerebrum, cerebellum and hypothalamus.
Indian J Exp Biol
;
2006 May; 44(5): 381-6
Article
in English
| IMSEAR
| ID: sea-62912
ABSTRACT
Acetylcholinesterase (AChE) has been purified from three different regions of rat brain using Sephadex G 200 column. SDS PAGE (6%) showed single band for the purified AChE fractions. Purified and lyophilized AChE from different (NH4)2SO4 precipitated fractions of three brain parts were utilized for in vitro enzyme kinetics using Dimethoate (Dmt) as inhibitor. K(m) values for cerebellum and hypothalamus were almost similar whereas cerebrum showed a different K(m) value compared to other two regions. With the drug Rivastigmine it was found that % G1 and G4 forms of AChE in three different parts of brain are different.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Acetylcholinesterase
/
Rats
/
Telencephalon
/
Male
/
Cerebellum
/
Chromatography, Ion Exchange
/
Electrophoresis, Polyacrylamide Gel
/
Hypothalamus
/
Animals
Language:
English
Journal:
Indian J Exp Biol
Year:
2006
Type:
Article
Similar
MEDLINE
...
LILACS
LIS