A partial sequence of lipoxygenase gene from genomic DNA of aromatic rice (Oryza sativa L.).
Indian J Exp Biol
;
2004 Feb; 42(2): 190-6
Article
in English
| IMSEAR
| ID: sea-63043
ABSTRACT
Aromatic (Bas-370, PB-1) and non-aromatic (Pusa-677, Pusa-834) rice were selected for the characterization and for distribution of lipoxygenase (Lox) genes. Polymorphism was observed when genomic DNA of rice varieties was hybridized with a heterologous lipoxygenase probe. A distinct polymorphic fragment (approximately 1.2 kb) was found in Bas-370. Sub-genomic library of Bas-370 was constructed and screened with LoxA probe. The smallest putative clone (pBas-14) of approximately 1.2 kb was sequenced. Complete nucleotide and deduced amino acid sequence showed the clone was 1134 bp long and comprised of 378 amino acid residues. PCR amplification of genomic DNA from four rice varieties with a soybean Lox primer also showed a polymorphic fragment of size approximately 600 bp (amplicon) in aromatic varieties that was sequenced directly. Nucleotide sequence alignment between pBas-14 and amplicon concluded that the amplicon was a part of the insert pBas-14.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Phylogeny
/
Plant Proteins
/
Polymorphism, Genetic
/
Oryza
/
Molecular Sequence Data
/
Base Sequence
/
DNA Probes
/
Genomic Library
/
Polymerase Chain Reaction
/
Amino Acid Sequence
Language:
English
Journal:
Indian J Exp Biol
Year:
2004
Type:
Article
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