Increased capacitation of buffalo sperm by heparin as confirmed by electron microscopy and in vitro fertilization.
Indian J Exp Biol
;
1997 Oct; 35(10): 1038-43
Article
in English
| IMSEAR
| ID: sea-63073
ABSTRACT
Capacitation of buffalo sperm was evaluated by induced acrosome reaction (AR) upon the exposure of 10 mM Ca2+. Culture of sperm for 8 hr in BO medium supplemented with 10 micrograms/ml heparin significantly (P < 0.01) increased the percentage of AR and confirmed by transmission electron microscopy. Vesiculization of outer acrosomal membrane and plasma membrane was observed significantly higher (P < 0.01) following 8 hr of sperm culture with heparin. Culture of sperm with heparin also increased rate of fertilization of in vitro matured oocytes and their subsequent development up to morula/blastocyst stage (P < 0.01). The study demonstrates that capacitation of buffalo sperm by heparin required at least 8 hr exposure of sperm to heparin for maximum acrosome reaction.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Sperm Capacitation
/
Male
/
Buffaloes
/
Heparin
/
Microscopy, Electron
/
Fertilization in Vitro
/
Animals
Language:
English
Journal:
Indian J Exp Biol
Year:
1997
Type:
Article
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