Use of meristem tip culture to eliminate carnation latent virus from carnation plants.
Indian J Exp Biol
;
2002 Jan; 40(1): 119-22
Article
in English
| IMSEAR
| ID: sea-63164
ABSTRACT
A successful protocol for meristem tip culture to eliminate carnation latent virus from carnation cv. scania has been described . The virus was found to be mechanically transmissible to Chenopodium quinoa, C. amaranticolor, Dianthus barbatus and Saponaria vaccaria. Murashige and Skoog'smedium (MS) supplemented with NAA (1.0 microM) and Kn (20.0 microM) proved best for meristem establishment and microshoots were rooted in MS medium supplemented with IBA (5.0 microM). Meristems measuring 0.1 and-0.2 mm yielded virus free plants and larger meristems were not effective.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Enzyme-Linked Immunosorbent Assay
/
Serologic Tests
/
Adenine
/
Carlavirus
/
Plant Shoots
/
Plant Leaves
/
Meristem
/
Dianthus
/
Alkaline Phosphatase
/
Kinetin
Type of study:
Diagnostic study
Language:
English
Journal:
Indian J Exp Biol
Year:
2002
Type:
Article
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