Cloning of 2.4 kb bovine herpesvirus-1 DNA fragment containing glycoprotein III gene into pUC18 plasmid vector.
Indian J Exp Biol
;
1995 Jun; 33(6): 459-61
Article
in English
| IMSEAR
| ID: sea-63400
ABSTRACT
A recombinant pBR322 plasmid containing bovine herpesvirus-1 HindIII 'I' fragment was analysed using EcoRI and BamHI restriction endonucleases. This recombinant plasmid was labelled with [alpha 32P]dATP and hybridized with southern blot of HindIII digested BHV-1 DNA fragments. A 2.4 kb double digested EcoRI-BamHI fragment of HindIII 'I' was subcloned into pUC18 plasmid to get complete gIII gene. The recombinant pUC18 plasmid was analysed for 2.4 kb BHV-1 DNA insert by restriction digestion with EcoRI and BamHI. Southern blot of restriction digested plasmid was hybridized with [alpha 32P]dATP labelled BHV-1 DNA probe.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Plasmids
/
Viral Proteins
/
DNA, Viral
/
Cattle
/
Deoxyribonuclease HindIII
/
Restriction Mapping
/
Cell Line
/
Cloning, Molecular
/
Herpesvirus 1, Bovine
/
Electrophoresis, Agar Gel
Language:
English
Journal:
Indian J Exp Biol
Year:
1995
Type:
Article
Similar
MEDLINE
...
LILACS
LIS