Nitrosomethylurea induced streptomycin resistance in Lycopersicon esculentum Mill.
Indian J Exp Biol
;
2000 Jun; 38(6): 617-20
Article
in English
| IMSEAR
| ID: sea-63463
ABSTRACT
High frequency of streptomycin resistant variants of Lycopersicon esculentum were isolated on selective shoot regeneration medium supplemented with IAA (0.5 mg/L), zeatin (1.5 mg/L) and streptomycin sulphate (500 mg/L). Nonmutagenized (controls) and NMU treated cotyledons were placed on shoot regeneration medium supplemented with antibiotic streptomycin. Resistant shoots appeared at a high frequency in mutagenized cotyledons, whereas in controls morphogenesis was suppressed, accompanied by bleaching. Shoot regeneration occurred from the nodular tissues developed at the cut ends of cotyledons. Resistant shoots developed into complete plantlets on rooting medium containing selective concentration of antibiotic. Stability of streptomycin resistance was confirmed by leaf assay and reciprocal crosses between streptomycin-resistant and sensitive plants.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Organ Culture Techniques
/
Seeds
/
Selection, Genetic
/
Zeatin
/
Breeding
/
Drug Resistance
/
RNA, Ribosomal
/
Streptomycin
/
Mutagenesis
/
Plastids
Language:
English
Journal:
Indian J Exp Biol
Year:
2000
Type:
Article
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