14C-urea breath test for assessment of gastric Helicobacter pylori colonization and eradication.
Article
in English
| IMSEAR
| ID: sea-63974
ABSTRACT
BACKGROUND AND OBJECTIVE:
Urea breath test (UBT) is a reliable noninvasive technique for detecting gastric Helicobacter pylori colonization. 14C isotope-based test requires simple equipment and is inexpensive. We studied the utility of 14C-UBT in diagnosis of gastric H. pylori infection.METHODS:
Presence of H. pylori was studied using antral histology and culture in patients with rapid urease test (RUT)-positive peptic ulcer. 14C-UBT was performed using a 185-kBq dose. Radioactivity in 15-min breath samples was measured using a beta-scintillation counter and result expressed as % dose recovered/mmol CO2. H. pylori was considered positive when any two tests were positive. All tests were repeated one month after completion of H. pylori eradication therapy.RESULTS:
Among 41 patients (duodenal ulcer 36, gastric ulcer 5), H. pylori was detected by histology in 23 (56%) and by culture in 27 (66%). Overall, H. pylori was detected in 28 (68%) patients. Follow-up assessment was possible in 28 patients 26 cleared the infection (all three tests negative). Mean 14C recovery values at 15 minutes associated with H. pylori-positive status were significantly higher (12.3 [SD 6.8] x 10(-3); n=30; p<0.001) than those associated with H. pylori-negative status (2.1 [0.9] x 10(-3); n=26). Using receiver-operating-characteristic analysis of 15-minute 14C recovery values, a cut-off of 6.5x10(-3) gave the best separation of H. pylori-positive and -negative cases. 14C-UBT had 93% sensitivity, 96% specificity and 95% accuracy.CONCLUSION:
14C-UBT appears to be a reliable noninvasive test for diagnosis of H. pylori infection.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Peptic Ulcer
/
Urea
/
Breath Tests
/
Female
/
Humans
/
Male
/
Carbon Radioisotopes
/
Predictive Value of Tests
/
ROC Curve
/
Follow-Up Studies
Type of study:
Diagnostic study
/
Observational study
/
Prognostic study
Language:
English
Year:
2001
Type:
Article
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