Coordinated secretion of alkaline phosphatase into serum and intestine in fat-fed rats.
Article
in English
| IMSEAR
| ID: sea-64211
ABSTRACT
BACKGROUND:
Fat feeding increases the activity of intestinal alkaline phosphatase in the serum. The mechanism underlying this increase is unknown. Surfactant-like particles (SLP) secreted by enterocytes have been implicated in this phenomenon.OBJECTIVE:
To study the effect of feeding fish oil and protein synthesis inhibitors on alkaline phosphatase activity in serum and in different intestinal fractions.METHODS:
Male albino rats were fed 2 mL of fish oil and were injected cycloheximide or actinomycin D. Alkaline phosphatase activity was determined in the serum and intestinal fractions (SLP, mucosa, muscularis).RESULT:
Feeding fish oil significantly elevated alkaline phosphatase activity in serum (p< 0.001) and intestinal mucosa (p< 0.01). Administration of cycloheximide or actinomycin D significantly reduced alkaline phosphatase activity in serum (p< 0.01) and in intestinal mucosa (p< 0.05). BCIP staining of brush border alkaline phosphatase activity in acrylamide gels yielded similar results.CONCLUSIONS:
These findings suggest a co-ordination between alkaline phosphatase synthesis and its assembly into lipoprotein vesicles, such as SLP, secreted by enterocytes in response to fat feeding.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Rats
/
Rats, Inbred Strains
/
Reference Values
/
Male
/
Dietary Fats
/
Metabolic Clearance Rate
/
Probability
/
Sensitivity and Specificity
/
Disease Models, Animal
/
Alkaline Phosphatase
Type of study:
Diagnostic study
/
Prognostic study
Language:
English
Year:
2004
Type:
Article
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