Isoenzyme and molecular characterization of Entamoeba histolytica and Entamoeba dispar isolates from symptomatic and asymptomatic subjects.
Article
in English
| IMSEAR
| ID: sea-64776
ABSTRACT
AIM:
To correlate the clinical features of amebic infections with the characteristics of Entamoeba culture isolates of stools.METHODS:
Isolates from seven irritable bowel syndrome (IBS) patients, four asymptomatic cyst passers (ACP) and five patients with invasive amebic disease were subjected to hexokinase polyacrylamide electrophoresis (HK-PAGE) and their DNA subjected to restriction fragment (RF) analysis of amplified polymerase chain reaction (PCR) products. These findings were correlated with anti-amebic serology. Two axenic pathogenic strains (HM1IMSS, NIH200) and one xenic nonpathogenic strain (SAW1734) were used as standards.RESULTS:
All isolates from IBS patients as well as ACP had slow-moving (nonpathogenic) band pattern, whereas those from patients with invasive disease had fast-moving (pathogenic) band pattern on HK-PAGE. Serological data using EIA and RF patterns of PCR-amplified genome corroborated these results.CONCLUSIONS:
Our results support the view that there are two species of Entamoeba infecting humans--E. histolytica(pathogenic) and E. dispar (nonpathogenic), and HK-PAGE of culture isolates can differentiate between them.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Polymorphism, Restriction Fragment Length
/
Humans
/
Inflammatory Bowel Diseases
/
DNA, Protozoan
/
Entamoeba
/
Entamoeba histolytica
/
Entamoebiasis
/
Hexokinase
/
Intestinal Diseases
/
Isoenzymes
Type of study:
Practice guideline
Language:
English
Year:
1999
Type:
Article
Similar
MEDLINE
...
LILACS
LIS