PD98059 inhibits expression of pERK1 protein and collagen alpha1(I) mRNA in rat pancreatic stellate cells activated by platelet-derived growth factor.
Article
in English
| IMSEAR
| ID: sea-65379
ABSTRACT
OBJECTIVE:
Pancreatic stellate cells (PSC) are considered as the principal effector cells in pancreatic fibrosis. We studied the role of platelet-derived growth factor (PDGF) in the activation of PSC.METHODS:
Cultured rat PSC were co-incubated with PDGF-BB (25 ng/mL) and different doses (0-40 ng/mL) of PD98059, a specific inhibitor of extracellular signal-regulated kinase (ERK). Expressions of p ERK1 protein and of collagen a1(I) mRNA were measured.RESULTS:
Expression of p ERK1 protein was up-regulated by PDGF-BB, and was down-regulated in a dose-dependent manner by PD98059. Expression of collagen a1(I) mRNA also showed an increase with PDGF-BB and non-dose-dependent inhibition by PD98059.CONCLUSION:
Our findings suggest that PSC activation is mediated by PDGF signal pathway, and ERK1 protein plays an important role in this activation.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Pancreas
/
Rats
/
Flavonoids
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Male
/
Platelet-Derived Growth Factor
/
RNA, Messenger
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Rats, Sprague-Dawley
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Calcium-Calmodulin-Dependent Protein Kinases
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EIF-2 Kinase
/
Reverse Transcriptase Polymerase Chain Reaction
Language:
English
Year:
2005
Type:
Article
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