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PD98059 inhibits expression of pERK1 protein and collagen alpha1(I) mRNA in rat pancreatic stellate cells activated by platelet-derived growth factor.
Article in English | IMSEAR | ID: sea-65379
ABSTRACT

OBJECTIVE:

Pancreatic stellate cells (PSC) are considered as the principal effector cells in pancreatic fibrosis. We studied the role of platelet-derived growth factor (PDGF) in the activation of PSC.

METHODS:

Cultured rat PSC were co-incubated with PDGF-BB (25 ng/mL) and different doses (0-40 ng/mL) of PD98059, a specific inhibitor of extracellular signal-regulated kinase (ERK). Expressions of p ERK1 protein and of collagen a1(I) mRNA were measured.

RESULTS:

Expression of p ERK1 protein was up-regulated by PDGF-BB, and was down-regulated in a dose-dependent manner by PD98059. Expression of collagen a1(I) mRNA also showed an increase with PDGF-BB and non-dose-dependent inhibition by PD98059.

CONCLUSION:

Our findings suggest that PSC activation is mediated by PDGF signal pathway, and ERK1 protein plays an important role in this activation.
Subject(s)
Full text: Available Index: IMSEAR (South-East Asia) Main subject: Pancreas / Rats / Flavonoids / Male / Platelet-Derived Growth Factor / RNA, Messenger / Rats, Sprague-Dawley / Calcium-Calmodulin-Dependent Protein Kinases / EIF-2 Kinase / Reverse Transcriptase Polymerase Chain Reaction Language: English Year: 2005 Type: Article

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Full text: Available Index: IMSEAR (South-East Asia) Main subject: Pancreas / Rats / Flavonoids / Male / Platelet-Derived Growth Factor / RNA, Messenger / Rats, Sprague-Dawley / Calcium-Calmodulin-Dependent Protein Kinases / EIF-2 Kinase / Reverse Transcriptase Polymerase Chain Reaction Language: English Year: 2005 Type: Article