In vitro studies on a bioreactor module containing encapsulated goat hepatocytes for the development of bioartificial liver.
Article
in English
| IMSEAR
| ID: sea-65469
ABSTRACT
BACKGROUND:
A bioartificial liver may act as a temporary metabolic bridge in patients with acute liver failure. We devised a bioreactor module containing encapsulated goat hepatocytes and studied its efficiency in detoxifying ammonia.METHODS:
A hollow-glass bioreactor module was designed and fabricated locally. The module was inoculated with alginate poly-L-lysine microcapsules with entrapped goat hepatocytes. Metabolism of ammonia and glucose was assessed. Antibody-mediated cell cytotoxicity was also assessed.RESULTS:
The optimum encapsulated goat hepatocyte concentration was 12-18 billion at a perfusate flow rate of 30 mL/min under oxygenated condition. The optimum ammonium chloride concentration for detoxification was 2.5-5.0 mM. There was little or no cytolysis of encapsulated hepatocytes on exposure to complement-inactivated human AB serum.CONCLUSION:
Encapsulated goat hepatocytes efficiently detoxified ammonia to urea. Cells were metabolically active up to 48 hours, indicating their feasibility for use in a bioreactor module. Encapsulation protected the hepatocytes from antibody-mediated cell lysis.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Urea
/
Humans
/
Goats
/
Bioreactors
/
Liver, Artificial
/
Hepatocytes
/
Ammonia
/
Animals
Language:
English
Year:
2002
Type:
Article
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