A monoclonal antibody-based test system for detection of Entamoeba histolytica-specific coproantigen.
Article
in English
| IMSEAR
| ID: sea-65641
ABSTRACT
BACKGROUND:
Diagnosis of amebiasis based on stool microscopy or demonstration of anti-amebic antibodies has limitations. A diagnostic system based on demonstration of the parasite product in clinical specimens holds promise.METHODS:
Murine monoclonal antibodies were developed against an Entamoeba histolytica-specific coproantigen. A monoclonal antibody (MoAb) 3D10 was employed in a double-antibody sandwich microELISA system for the detection of amebic coproantigen in fecal specimens. The system was evaluated in three groups ofsubjects:
63 patients with intestinal amebae, 27 with non-amebic parasitosis, and 57 apparently healthy controls.RESULTS:
The MoAb 3D10 belonged to IgG1 isotype and recognized three antigens, with mol. wt. 36, 25 and 17 kDa in the crude extract of E. histolytica (HM1-IMSS), and an amebic coproantigen with MW 36 kDa in the stool supernatant from patients with intestinal amebae. The coproantigen was detected in the stool eluates of 56 (89%) patients with intestinal amebae and in none of the stool eluates from other subjects, thereby giving this system a sensitivity of 89% and specificity of 100% for the detection of intestinal amebae.CONCLUSIONS:
This monoclonal antibody recognizes as intact epitope on the E. histolytica-specific coproantigen. The validity of the MoAb-based microELISA system needs to be established.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Female
/
Humans
/
Male
/
Enzyme-Linked Immunosorbent Assay
/
Case-Control Studies
/
Sensitivity and Specificity
/
Entamoeba histolytica
/
Entamoebiasis
/
Feces
/
Animals
Type of study:
Diagnostic study
/
Observational study
Language:
English
Year:
1999
Type:
Article
Similar
MEDLINE
...
LILACS
LIS