Carbachol potentiates cholera toxin-induced secretion in a colonic epithelial cell line (HT29-19A) and rat ileal mucosa in vitro.
J Health Popul Nutr
;
2000 Jun; 18(1): 49-53
Article
in English
| IMSEAR
| ID: sea-670
ABSTRACT
Recent studies show that enteric nerves are involved in the action of cholera toxin, both in vivo and in vitro. The aim of this study was to investigate in vitro the influence of carbachol, a cholinergic agonist, on the action of cholera toxin. Cultured HT29-19A cell lines and rat ileal mucosa were used in an Ussing chamber for the measurement of short-circuit current induced by cholera toxin. Cyclic AMP was measured from HT29-19A cell lines by standard radio-immunoassay. Pre-treatment of the HT29-19A cell lines with carbachol potentiated cholera toxin-induced secretory response, and enhanced accumulation of cAMP. Carbachol also potentiated the cholera toxin-secretory response in the rat ileal mucosa, but only following pretreatment with the prostaglandin synthesis inhibitor, indomethacin. There was synergistic interaction between cholera toxin and cholinergic neurotransmitter carbachol on the intestinal epithelium. Cholinergic agonists may play a role in regulating the secretory response to the toxin. Such interaction is masked in the intact tissues in vitro due to the release of prostaglandins during isolation.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Rats
/
Humans
/
Male
/
Carbachol
/
Indomethacin
/
Cholera Toxin
/
Cyclooxygenase Inhibitors
/
Rats, Sprague-Dawley
/
Cyclic AMP
/
Cholinergic Agonists
Language:
English
Journal:
J Health Popul Nutr
Journal subject:
Gastroenterology
/
Nutritional Sciences
/
Public Health
Year:
2000
Type:
Article
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