Usefulness of CD45 density in the diagnosis of B-cell chronic lymphoproliferative disorders.
Indian J Med Sci
;
2005 May; 59(5): 187-94
Article
in English
| IMSEAR
| ID: sea-67118
ABSTRACT
BACKGROUND:
Although many B-cell chronic lymphoproliferative disorders (BCLPDs) including B-cell chronic lymphocytic leukemia (B-CLL) have characteristic clinical and biological features, the overlapping morphologic and immunophenotypic profiles of various BCLPDs, is still the main problem.AIM:
Our aim was to evaluate the usefulness of CD45 expression in the immunological classification of BCLPDs. SETTING ANDDESIGN:
A prospective study was set in a university hospital to investigate the CD45 intensity, particularly in B-CLL. MATERIALS ANDMETHODS:
The expression of CD45 in 37 patients with BCLPD including typical B-CLL (Group I), atypical B-CLL and CLL/PLL (II), and hairy cell leukemia (HCL), B-prolymphocytic leukemia (B-PLL), and B-non Hodgkin's lymphoma (B-NHL) as non-CLL BCLPDs (III) and in eight healthy age matched controls (IV) was quantitatively compared by flow cytometric CD45/RALS gating strategy. Statisticalanalysis:
The mean, median, and peak channel scores of CD45 obtained for the four groups were compared using one-way analysis of variance test. A P valueRESULTS:
Lower CD45 density is associated highly with typical CLL and differences between typical CLL and other groups were significant (P< 0.001, 0.001, and 0.001). Non-CLL cases had significantly brighter CD45 expression than atypical CLL (P=0.014). No differences were found between normal lymphocytes and non-CLL BCLPD cases.CONCLUSIONS:
CD45 is a useful marker, to discriminate the typical CLL from the non-CLL BCLPD and from atypical CLL.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Phosphoproteins
/
Aged, 80 and over
/
Aged
/
Female
/
Humans
/
Male
/
B-Lymphocytes
/
Biomarkers
/
Leukemia, B-Cell
/
Prospective Studies
Type of study:
Diagnostic study
/
Observational study
/
Prognostic study
/
Risk factors
Limits:
Aged80
Language:
English
Journal:
Indian J Med Sci
Year:
2005
Type:
Article
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