Influence of UV-irradiation on enzymes in mouse ocular lens: in vitro studies.
Indian J Ophthalmol
;
1999 Mar; 47(1): 25-9
Article
in English
| IMSEAR
| ID: sea-70927
ABSTRACT
PURPOSE:
In vitro study of the enzymes involved in aerobic, anaerobic and hexose monophosphate shunt in ultraviolet radiation exposed mice lenses.METHOD:
Of the selected enzymes, lactic dehydrogenase (LDH) was representative of anaerobic glucose oxidation, succinic dehydrogenase (SDH) of the aerobic oxidation, and Glucose-6-phosphate dehydrogenase (G-6-PDH) of the Hexose Monophosphate (HMP) shunt. Other enzymes studied were ATPase and glutathione reductase (GR).RESULTS:
Experiments with mice lenses in vitro showed that transparent lens became opaque following UV-irradiation at 360 nm. Opacification of the lens was accompanied by a change in enzyme activities for energy metabolism.CONCLUSION:
These changes were progressive in a manner analogous to sequential morphological changes, which would be crucial in maintaining lens transparency.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Ultraviolet Rays
/
Cataract
/
Energy Metabolism
/
Enzymes
/
Lens, Crystalline
/
Animals
/
Mice
Language:
English
Journal:
Indian J Ophthalmol
Year:
1999
Type:
Article
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