The effect of infliximab, cyclosporine A and recombinant IL-10 on vitreous cytokine levels in experimental autoimmune uveitis.
Indian J Ophthalmol
;
2006 Dec; 54(4): 241-5
Article
in English
| IMSEAR
| ID: sea-71034
ABSTRACT
BACKGROUND:
To identify the effect of infliximab, cyclosporine A and recombinant IL-10 in experimental autoimmune uveitis. MATERIALS ANDMETHODS:
Sixty male rats were assigned to five groups of 12 each. All the groups (except the control group) were administered 30 microg retinal-S antigen intraperitoneally. On the 14th day, after confirmation of uveitis with histopathological study, daily cyclosporine A injection was given in cyclosporine A treatment group and physiological serum in the uveitis-induced placebo treatment and control groups. In the infliximab treatment group, infliximab was administered on the 14th, 15th, 17th, 19th and 21st days. In the recombinant IL-10 treatment group, three doses of recombinant IL-10 were given four hours and a half hours before and eight hours after retinal-S antigen administration. On the 21st day of the study, all rats were sacrificed and vitreous cytokine levels (IL-1, IL-6, IL-8 and TNF-alpha) were studied with ELISA.RESULTS:
In the treatment groups, cytokine levels (IL-1, IL-6 and TNF-alpha) were significantly lower than the uveitis-induced placebo treatment group. Compared with the control group, there was no significant difference with respect to TNF-alpha and IL-8 in the infliximab treatment group; IL-8 in the cyclosporine A treatment group; IL-6 and IL-8 in the recombinant IL-10 treatment group. The drugs used did not significantly differ in respect to their effects on vitreous IL-6, IL-8 and TNF-alpha levels.CONCLUSION:
Cyclosporine A, infliximab and recombinant IL-10 reduce the vitreous cytokines levels. Among these drugs, recombinant IL-10, which is still in its experimental phase, might be considered as a new therapeutic agent.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Rats
/
Rats, Inbred Lew
/
Autoimmune Diseases
/
Uveitis
/
Vitreous Body
/
Recombinant Proteins
/
Male
/
Biomarkers
/
Follow-Up Studies
/
Cytokines
Type of study:
Observational study
/
Prognostic study
Language:
English
Journal:
Indian J Ophthalmol
Year:
2006
Type:
Article
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