Rapid detection of fungal filaments in corneal scrapings by microwave heating-assisted Grocott's methenamine silver staining.

Vemuganti, Geeta K; Naidu, Chenchu; Gopinathan, Usha

Vemuganti, Geeta K; Naidu, Chenchu; Gopinathan, Usha.

Indian J Ophthalmol ; 2002 Dec; 50(4): 326-8

Article in English | IMSEAR | ID: sea-71209

ABSTRACT

ABSTRACT

The Gomoris methanamine silver impregnation technique is a highly reliable and archiveable method of detecting fungal filaments, but the staining procedure is time consuming and laborious. A technique using microwave energy to reduce the duration of Gomori's silver staining is described.

Subject(s)

Cornea/microbiology; Eye Infections, Fungal/diagnosis; Fungi/isolation & purification; Heating/methods; Humans; Keratitis/diagnosis; Microwaves; Mycoses/diagnosis; Sensitivity and Specificity; Silver Staining/instrumentation; Staining and Labeling/methods

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Full text: Available Index: IMSEAR (South-East Asia) Main subject: Staining and Labeling / Humans / Eye Infections, Fungal / Sensitivity and Specificity / Silver Staining / Cornea / Fungi / Heating / Keratitis / Microwaves Type of study: Diagnostic study Language: English Journal: Indian J Ophthalmol Year: 2002 Type: Article

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