Changes in the corneal Na-K ATPase levels in eyes stored in moist chamber at 4 degrees C.
Indian J Ophthalmol
;
1996 Mar; 44(1): 15-8
Article
in English
| IMSEAR
| ID: sea-72540
ABSTRACT
This report deals with a chronological measurement of Na-K ATPase enzyme activity in human and bovine corneas stored in a moist chamber at 4 degrees C. Paired human and bovine eyes were sterilized by the standard eye bank procedure and stored up to 6 days. At the desired time, the corneal endothelium was assayed for Na-K ATPase activity. The protein content of each tissue sample was also determined. In a parallel set of experiments, the viability of identical stored corneas was determined by trypan blue and alizarin red staining technique, and morphometric analysis was done to quantify the extent of the corneal endothelial damage. The human corneas showed that there was a significant progressive decrease in the Na-K ATPase activity as the storage time increased. The decrease was related to morphological endothelial damage.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Organ Preservation
/
Time Factors
/
Humans
/
Cattle
/
Endothelium, Corneal
/
Cryopreservation
/
Cell Survival
/
Analysis of Variance
/
Sodium-Potassium-Exchanging ATPase
/
Cornea
Language:
English
Journal:
Indian J Ophthalmol
Year:
1996
Type:
Article
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