Laboratory methods in the detection of herpes simplex virus (HSV) in keratitis--a 9-year study including polymerase chain reaction (PCR) during last 4 years.
Indian J Pathol Microbiol
;
2003 Jan; 46(1): 109-12
Article
in English
| IMSEAR
| ID: sea-73232
ABSTRACT
Conventional methods of fluorescent antibody test (FAT) and virus isolation (VI) and molecular method of polymerase chain reaction (PCR) were compared for the detection of HSV in keratitis during a 9-year period. Of 186 corneal scraping specimens, 108 were subjected to FATand VI in the pre-PCR period (initial 5 years) while 78 to FAT, VI and PCR in the PCR period (latter 4 years). HSV was detected by FAT in 44/186 (23.7%), VI in 18/186 (9.7%) and PCR in 27/78 (34.6%) specimens. Overall, HSV was diagnosed in 56/186 (30.1%) specimens. PCR has increased the clinical sensitivity by 12.8%, which is statistically significant (McNemar test, P-0.002). VI should be replaced by PCR. FAT though less sensitive should always be employed as a routine to give an early diagnosis, the results of which could be further confirmed, if necessary, by PCR, which is a more sensitive and specific diagnostic tool.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Humans
/
Polymerase Chain Reaction
/
Simplexvirus
Type of study:
Diagnostic study
/
Screening study
Language:
English
Journal:
Indian J Pathol Microbiol
Year:
2003
Type:
Article
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