Detection of extended spectrum beta-lactamase from clinical isolates in Davangere.
Indian J Pathol Microbiol
;
2008 Oct-Dec; 51(4): 497-9
Article
in English
| IMSEAR
| ID: sea-73356
ABSTRACT
Extended spectrum beta-lactamase enzymes (ESBLs) are enzymes that have the ability to hydrolyze oxyiminocephalosporins and infections by isolates producing them are often difficult to treat. A study to detect the presence of these enzymes in isolates was conducted by our hospital. A total of 207 non repetitive isolates were screened for resistance to any of five screening agents. Those with suspicious profiles were checked for ESBL production by double-disk approximation or a synergy test. The isolates were also subjected to a phenotypic confirmation test as recommended by CLSI (formerly NCCLS). Various cephalosporins-beta-lactamase inhibitor combinations were also tested. Of the 204 (98.5%) screen-positive isolates, only 126 (61.7%) were identified as ESBL producers. Of these, 26.1% of the isolates were positive by using the double-disk synergy test (DDST) method alone, 13.4% were positive using the method recommended by CLSI, and 60.3% of the isolates were positive by both the DDST and CLSI methods. We also report a high percentage of resistance to cefoxitin (96.8%) indicating changes in porins.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Beta-Lactamases
/
Female
/
Humans
/
Male
/
Microbial Sensitivity Tests
/
Cephalosporins
/
Cephalosporin Resistance
/
Beta-Lactam Resistance
/
Beta-Lactams
/
Enterobacteriaceae
Type of study:
Diagnostic study
Language:
English
Journal:
Indian J Pathol Microbiol
Year:
2008
Type:
Article
Similar
MEDLINE
...
LILACS
LIS