Sequence analysis of bla CTX-M-28 , an ESBL responsible for third-generation cephalosporin resistance in Enterobacteriaceae, for the first time in India.
Indian J Pathol Microbiol
;
2008 Apr-Jun; 51(2): 218-21
Article
in English
| IMSEAR
| ID: sea-73806
ABSTRACT
The most common group of ESBLs not belonging to the bla TEM or bla SHV families were termed bla CTX-M , to highlight their ESBLs' greater activity against cefotaxime than against ceftazidime. The presence of nosocomial bla CTX-M-28 -producing Enterobacteriaceae strains has not been reported earlier in Indian hospitals. The sequences of bla CTX-M-28 gene from cephalosporin-resistant Enterobacteriaceae were analyzed. The structural gene encodes a 290 amino-acid protein, which is most related to the bla CTX-M beta-lactamases. The conserved K-T-G was identified in the bla CTX-M-28 protein sequence, but significantly, two point mutations (N-->T) and (F-->S) were identified in the Y-G-N- and S-T-F-K-conserved motifs respectively. These point mutations were seen in all the three sequenced isolates.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Beta-Lactamases
/
Humans
/
DNA, Bacterial
/
Molecular Sequence Data
/
Base Sequence
/
Cephalosporin Resistance
/
Enterobacter
/
Enterobacteriaceae
/
Enterobacteriaceae Infections
/
Escherichia coli
Country/Region as subject:
Asia
Language:
English
Journal:
Indian J Pathol Microbiol
Year:
2008
Type:
Article
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