Identification of C-3 on polyacrylamide gel by pre-staining the normal human serum with Remazol Brilliant Blue and raising monospecific anti C-3 antibody.
Indian J Pathol Microbiol
;
1989 Jul; 32(3): 221-6
Article
in English
| IMSEAR
| ID: sea-74180
ABSTRACT
Based on our earlier work we hereby describe pre-staining of normal human serum with Remazol Brilliant Blue (RBB) to precisely locate the third component of complement system (C3,B1C) on polyacrylamide gels. Testing the dye-protein complex eluted from different immunoreactive post-transferrin (origin to transferrin) components against the standards anti-C3 antibody in various gel precipitation techniques, revealed that the prominent band just posterior to the centrally placed transferrin is of complement. Subsequent to its identity, we also evolved a method to raise an anti-C3 antibody. The resulting antibody was found to be monospecific when assessed in different gel precipitation procedures.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Humans
/
Complement C3
/
Anthraquinones
/
Coloring Agents
/
Electrophoresis, Polyacrylamide Gel
/
Antibodies
Language:
English
Journal:
Indian J Pathol Microbiol
Year:
1989
Type:
Article
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