Incidence of lamivudine resistance associated mutations in pol-gene of HIV-1 in patients from north India: a preliminary report.
Indian J Pathol Microbiol
;
2005 Jul; 48(3): 337-40
Article
in English
| IMSEAR
| ID: sea-74485
ABSTRACT
Screening of drug-resistant variants is very important for the effective clinical management of HIV-infected patients and development of new strategies. The present study was aimed to detect codon-184 mutations in the pol-gene of HIV leading to resistance to lamivudine (3-TC) by nested cum ARMS-PCR approach in 10 treated and 9 treatment naive patients. For correlation the whole blood CD4/CD8 cell counts and the soluble TNFRII levels in plasma were also determined. Of the 19 patients tested, mutant variants were observed in 2 patients (Met Val in one and Met Val & lle in second) both being treated with 3-TC. No mutations were detected in the treatment-naive patients. The results confirmed that, drug resistant variants of codon-184 emerge rapidly in patients receiving 3-TC containing regimens including our population, which is mainly infected with subtypeC of the virus that could be detected along with wild viral population using sensitive approaches such as ARMS-PCR.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Humans
/
Codon
/
Genes, pol
/
HIV Infections
/
Incidence
/
HIV-1
/
CD4 Lymphocyte Count
/
Reverse Transcriptase Inhibitors
/
Lamivudine
/
Anti-HIV Agents
Type of study:
Incidence study
/
Prognostic study
Country/Region as subject:
Asia
Language:
English
Journal:
Indian J Pathol Microbiol
Year:
2005
Type:
Article
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