Diagnosis of tuberculosis by polymerase chain reaction.
Indian J Pediatr
;
1990 Sep-Oct; 57(5): 673-7
Article
in English
| IMSEAR
| ID: sea-79290
ABSTRACT
For the diagnosis of extrapulmonary tuberculosis in adults and all forms of tubercular infections in children, microscopic and cultural techniques have been shown to be inadequate. Many serological techniques have been employed for non culture diagnosis of tuberculosis. Early promising results have repeatedly given way to subsequent findings of non-specificity. Major mycobacterial antigens have been shown to be heat shock proteins which are highly conserved in nature. DNA probes for tuberculosis are specific but have a sensitivity equivalent to AFB smear examination. Polymerase Chain Reaction (PCR) with its ability to selectively amplify DNA fragments of interest offers a potentially powerful technique for the rapid, specific and sensitive diagnosis of tuberculosis. Samples from partially treated patients could be culture negative but can be detected by PCR.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Tuberculosis
/
Humans
/
DNA Probes
/
Cells, Cultured
/
Child
/
Polymerase Chain Reaction
/
Adult
/
Mycobacterium tuberculosis
Language:
English
Journal:
Indian J Pediatr
Year:
1990
Type:
Article
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