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VP16CREB-induced differentiation of neuroblastoma.
Indian J Pediatr ; 2008 Oct; 75(10): 1009-13
Article in English | IMSEAR | ID: sea-83045
ABSTRACT

OBJECTIVE:

Drug-induced differentiation is commonly used as a therapeutic modality for the treatment of neuroblastoma tumors. Increased level of cyclic adenosine 3', 5'-monophosphate (cAMP) mediates terminal differentiation in some neuroblastoma cell lines through activation of several signaling networks, including cAMP response element binding protein (CREB). Objective was to test whether cAMP-induced differentiation in a murine neuroblastoma cell line (NBP2) is partly mediated by CREB.

METHODS:

Fluorescent microscopy was used to document neuron-like morphological changes imparted by a constitutively active CREB (VP16CREB). Real time PCR (RT-PCR) was performed to verify changes in the expression of cAMP/CREB responsive genes.

RESULTS:

It was found that transient expression of VP16CREB into NBP2 cells resulted in morphological changes that were characteristics of terminally differentiated neurons. Furthermore, increased expression of cAMP responsive genes was compromised in cells resisting VP16CREB-mediated differentiation.

CONCLUSION:

A constitutively active CREB induces terminal differentiation in a subset of NBP2 cell population. Altered expression of cAMP responsive genes may account for differentiation resistant phenotype in NBP2 cells.
Subject(s)
Full text: Available Index: IMSEAR (South-East Asia) Main subject: Tumor Cells, Cultured / Signal Transduction / Receptors, Steroid / Gene Expression / Cell Cycle / Cell Differentiation / Polymerase Chain Reaction / Herpes Simplex Virus Protein Vmw65 / Cyclic AMP Response Element-Binding Protein / Cyclic AMP Language: English Journal: Indian J Pediatr Year: 2008 Type: Article

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Full text: Available Index: IMSEAR (South-East Asia) Main subject: Tumor Cells, Cultured / Signal Transduction / Receptors, Steroid / Gene Expression / Cell Cycle / Cell Differentiation / Polymerase Chain Reaction / Herpes Simplex Virus Protein Vmw65 / Cyclic AMP Response Element-Binding Protein / Cyclic AMP Language: English Journal: Indian J Pediatr Year: 2008 Type: Article