Detection of Entamoeba histolytica DNA in the saliva of amoebic liver abscess patients who received prior treatment with metronidazole.
J Health Popul Nutr
;
2008 Dec; 26(4): 418-25
Article
in English
| IMSEAR
| ID: sea-926
ABSTRACT
Saliva is an easily-accessible and a non-invasive clinical specimen alternate to blood and liver pus. An attempt was made to detect Entamoeba histolytica DNA released in the saliva of amoebic liver abscess (ALA) patients by applying 16S-like rRNA gene-based nested multiplex polymerase chain reaction (NM-PCR). The NM-PCR detected E. histolytica DNA in the saliva of eight (28.6%) of 28 ALA patients. The NM-PCR result was negative for E. histolytica DNA in the saliva of all the eight ALA patients who were tested prior to treatment with metronidazole but was positive in the saliva of eight (40%) of 20 ALA patient who were tested after therapy with metronidazole. The NM-PCR detected E. histolytica DNA in liver abscess pus of all 28 (100%) patients with ALA. The TechLab E. histolytica II enzyme-linked immunosorbent assay was positive for E. histolytica Gal/GalNAc lectin antigen in the liver abscess pus of 13 (46.4%) of the 28 ALA patients. The indirect haemagglutination (IHA) test was positive for anti-amoebic antibodies in the serum of 22 (78.6%) of the 28 ALA patients and 2 (5.7%) of 35 healthy controls. The present study, for the first time, demonstrates the release of E. histolytica DNA in the saliva of ALA patients by applying NM-PCR.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Saliva
/
Humans
/
Enzyme-Linked Immunosorbent Assay
/
Antibodies, Protozoan
/
Polymerase Chain Reaction
/
DNA, Protozoan
/
Entamoeba histolytica
/
India
/
Liver Abscess, Amebic
/
Animals
Type of study:
Diagnostic study
Country/Region as subject:
Asia
Language:
English
Journal:
J Health Popul Nutr
Journal subject:
Gastroenterology
/
Nutritional Sciences
/
Public Health
Year:
2008
Type:
Article
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