Study on gene polymorphism of multiple RBC blood group systems in Mongolian population in Inner Mongolia / 中国输血杂志

ABSTRACT

【Objective】 To investigate the gene frequency and polymorphism of 12 RBC blood group systems, including RHCE, Lw, Duffy, Kidd, MNS, Scianna, Colton, Dombrock, Kell, Diego, Yt, and Lutheran blood group systems in Mongolian in Inner Mongolia, so as to provide data for the establishment of rare blood group registry in this region. 【Methods】 Twelve blood groups of 220 Mongolian people in Inner Mongolia were genotyped and analyzed by Fluo-PCR. 【Results】 The genes frequency of the 12 rare blood group was as follows 1)RhCE, C=0.613 6, c=0.386 4, E=0.265 9, e=0.734 1; MNS, M=0.609 1, N=0.390 9, S=0.063 6, s=0.931 8, Mur=0; Duffy, Fya=0.856 8, Fyb=0.143 2; Kidd, Jka=0.522 7, Jkb=0.477 3; Diego, Dia=0.027 3, Dib=0.972 7, Wra=0, Wrb=1; Dombrock, Doa=0.163 6, Dob=0.836 4. 2) Kell, K=0.002 3, k=0.997 7, Kpa=0.009 1, Kpb=0.990 9; Yt, Yta=0.986 4, Ytb=0.013 6. 3) Lw, Lwa=1, Lwb=0; Sc2=0; Colton, Coa=1, Cob=0; Lutheran, Lua=0, Lub=1. The 220 Mongolian people with Lw, Scianna, Colton and Lutheran were all homozygous, and their genotypes were Lwa/Lwa, Sc1/Sc1, Lub/Lub and Coa/Coa, respectively. 【Conclusion】 The RHCE, MNS, Duffy, Kidd, Diego and Dombrock blood types of Mongolian population in Inner Mongolia are polymorphic with certain distribution characteristics. The MNS blood group system does not conform to the Hardy-Weinberg equilibrium(P<0.05), which may be related to the sample size or genetic changes. Kell, Lw, Scianna, Colton, Yt and Lutheran showed a monomorphic distribution.