Influencing factors of platelet-rich plasma collection / 中国输血杂志

ABSTRACT

【Objective】 To explore the factors affecting the collection and product quality of platelet-rich plasma (PRP), and to provide reference for ensuring the safety of PRP collection and the quality of PRP products. 【Methods】 A total of 108 patients receiving PRP treatment in our hospital from February 20 to April 20, 2016 were investigated by analyzing the time of PRP collection, the amount of circulating blood, the amount of anticoagulant and the use of calcium from four factors including gender, age, Plt before collection and collection equipment. The effects of PRP product volume, platelet content, RBC and WBC contamination, and the occurrence of adverse reactions were statistically analyzed. 【Results】 The type of equipment had little influence on the collection parameters and product quality(P>0.05). RBC residues in female patients were (0.07±0.03) ×1012/L, higher than that in male patients (0.04±0.02)×1012/L (P<0.05). Significant differences in collection time, circulating blood volume, anticoagulant dosage and collection volume were noticed by age(18~40 vs 56~77 years old, P<0.05) and platelet count(×109/L) (201~450 vs 100~150, P<0.05). In patients with low Plt count (100~150)×109/L before collection, the Plt content in PRP product was relatively low(771±218)×109/L, and WBC(0.45±0.25)×109/L and RBC(0.07±0.03)×1012/L residual amount was rather high(P<0.05), but also satisfied the quality criteria. 【Conclusion】 The MCS+ and NGL XCF 3000 blood cell separators are safe and stable in PRP collection, even in patients with low platelet count(100~150)×109/L or old age (>55 years old).