Testosterone inhibits eryptosis induced by hydrogen peroxide / 中国输血杂志

ABSTRACT

【Objective】 To explore the mechanism of testosterone on eryptosis. 【Methods】 The erythrocyte suspension (1%) was cultured in vitro and divided into 3 groups 2 kinds of eryptosis models induced by hydrogen peroxide (H2O2) in vitro, 3×10-8 mol/L testosterone + 200 μmol/L H2O2 group (group A), 200 μmol/L H2O2 treatment group (group B) and 1×PBS buffer control group (group C). Erythrocytes were collected from 12 well plates (about 2×106/well) at 24 h and 60 h to detect the eryptosis rate, intracellular ROS and [Ca2+ ]i through flow cytometry, and the changes of each index were analyzed by t test. 【Results】 The eryptosis rate(%)at 24 h and 60 h in group A, B and C were 2.61±0.28, 11.25±1.43 vs 7.15±0.95, 28.65±0.74 vs 1.32±0.07, 8.18±0.08 (P<0.01); ROS level(%) 14.52±0.68, 15.26±0.49 vs 16.68±0.60, 21.68±1.10 vs 7.61±0.21, 10.29±1.06 (P<0.01); [Ca2+ ]i(%) 6.54±0.46, 8.93±0.87 vs 11.78±0.76, 14.63±0.80 vs 1.36±0.20, 2.44±0.38 (P<0.01). The eryptosis rate, ROS level and [Ca2 + ]i in group A were significantly lower than those in group B (P<0.01). 【Conclusion】 Testosterone effectively inhibits eryptosis induced by H2O2 in vitro and its mechanism may be related to reducing ROS production and maintaining normal [Ca2+ ]i.