Effect of storage duration of whole blood on the preparation of leukocyte-depleted red blood cells / 中国输血杂志

ABSTRACT

【Objective】 To discuss the differences in the quality of leukocyte-depleted red blood cells(LDRBCs) prepared from fresh whole blood stored at 4℃ with different storage duration. 【Methods】 The whole blood was collected and stored at 4℃. LDRBCs were prepared on the day of collection (d0 preparation group) and the next day (d2 preparation group). The leukocyte residues, blood filtering time, blood loss during filtration, LDRBCs volume, and the Hct, MCV, RBC and Hb in the packed whole blood with different storage duration, and the K+, Na+, pH, free hemoglobin concentration(fHb) and hemolysis rate of plasma between two groups were analyzed and compared. 【Results】 The filtering time of d0 preparation group and d2 preparation group was (7.67±1.95) min and (11.55±2.43) min, respectively. The volume of LDRBCs in the two groups was (305.21±21.95) mL and (311.11±21.08) mL, respectively (P<0.05). Concerning blood loss during filtration and WBC residues, there was no difference between two groups (P>0.05). No significant differences could be noticed in the value of Hct, RBC and Hb during the storage period(P>0.05), irrespective of MCV(fL), which changed significantly when sampled immediately after blood collection (89.37±2.57), prior to preparation at day 0(89.68±2.74) and prior to preparation at day 2(90.30±3.25) (P<0.05). No difference in K+, Na+, pH value and fHb was notable between the two groups (P>0.05), while hemolysis rate of plasma in day 0 preparation group was lower than that of day 2 preparation group (P<0.05). 【Conclusion】 The RBC deformability, increasing with the extension of whole blood cold storage time, results in the differences in the volume of LDRBCs and incidence of hemolysis. LDRBCs preparation within 10-hour cold storage following whole blood collection is recommended.