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Identification of a new C-23 metabolite in sterol degradation of Mycobacterium neoaurum HGMS2 and analysis of its metabolic pathways / 生物工程学报
Chinese Journal of Biotechnology ; (12): 4550-4562, 2023.
Article in Chinese | WPRIM | ID: wpr-1008041
ABSTRACT
Mycobacterium neoaurum has the ability to produce steroidal intermediates known as 22-hydroxy-23, 24-bisnorchol-4-en-3-one (BA) upon the knockout of the genes for either the hydroxyacyl-CoA dehydrogenase (Hsd4A) or acyl-CoA thiolase (FadA5). In a previous study, we discovered a novel metabolite in the fermentation products when the fadA5 gene was deleted. This research aims to elucidate the metabolic pathway of this metabolite through structural identification, homologous sequence analysis of the fadA5 gene, phylogenetic tree analysis of M. neoaurum HGMS2, and gene knockout. Our findings revealed that the metabolite is a C23 metabolic intermediate, named 24-norchol-4-ene-3, 22-dione (designated as 3-OPD). It is formed when a thioesterase (TE) catalyzes the formation of a β-ketonic acid by removing CoA from the side chain of 3, 22-dioxo-25, 26-bisnorchol-4-ene-24-oyl CoA (22-O-BNC-CoA), followed by spontaneously undergoing decarboxylation. These results have the potential to contribute to the development of novel steroid intermediates.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Phylogeny / Steroids / Sterols / Metabolic Networks and Pathways / Mycobacterium Language: Chinese Journal: Chinese Journal of Biotechnology Year: 2023 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Phylogeny / Steroids / Sterols / Metabolic Networks and Pathways / Mycobacterium Language: Chinese Journal: Chinese Journal of Biotechnology Year: 2023 Type: Article