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Adaptation of the electron transport chain improves the biocatalytic efficiency of progesterone 17α hydroxylation / 生物工程学报
Chinese Journal of Biotechnology ; (12): 4608-4620, 2023.
Article in Chinese | WPRIM | ID: wpr-1008045
ABSTRACT
17α hydroxylase is a key enzyme for the conversion of progesterone to prepare various progestational drug intermediates. To improve the specific hydroxylation capability of this enzyme in steroid biocatalysis, the CYP260A1 derived from cellulose-mucilaginous bacteria Sorangium cellulosum Soce56 and the Fpr and bovine adrenal-derived Adx4-108 derived from Escherichia coli str. K-12 were used to construct a new electron transfer system for the conversion of progesterone. Selective mutation of CYP260A1 resulted in a mutant S276I with significantly enhanced 17α hydroxylase activity, and the yield of 17α-OH progesterone reached 58% after optimization of the catalytic system in vitro. In addition, the effect of phosphorylation of the ferredoxin Adx4-108 on 17α hydroxyl activity was evaluated using a targeted mutation technique, and the results showed that the mutation Adx4-108T69E transferred electrons to S276I more efficiently, which further enhanced the catalytic specificity in the C17 position of progesterone, and the yield of 17α-OH progesterone was eventually increased to 74%. This study provides a new option for the production of 17α-OH progesterone by specific transformation of bacterial-derived 17α hydroxylase, and lays a theoretical foundation for the industrial production of progesterone analogs using biotransformation method.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Progesterone / Electron Transport / Biocatalysis / Mixed Function Oxygenases / Hydroxylation Limits: Animals Language: Chinese Journal: Chinese Journal of Biotechnology Year: 2023 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Progesterone / Electron Transport / Biocatalysis / Mixed Function Oxygenases / Hydroxylation Limits: Animals Language: Chinese Journal: Chinese Journal of Biotechnology Year: 2023 Type: Article