Structural characterization of PCP-Ⅰ from Poria as vaccine adjuvant and its hydrolytic oligosaccharide / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 4429-4437, 2023.
Article
in Chinese
| WPRIM
| ID: wpr-1008697
ABSTRACT
Poria is an important medical herb in clinic. The authors isolated a polysaccharide(PCP-Ⅰ) from Poria in previous studies, which is composed of galactose, mannose, fucose and glucose. PCP-Ⅰ exhibited significant adjuvant effects on H1N1 influenza vaccine, hepatitis B surface antigen and anthrax protective antigen, and its adjuvant activity was stronger than aluminium adjuvant. However, little is known about the chemical structure of PCP-Ⅰ at present. In this study, weak acid hydrolysis was used to obtain the backbone oligosaccharide of PCP-Ⅰ. Then periodate oxidation, Smith degradation, methylation analysis, Fourier transform infrared spectroscopy(FT-IR), nuclear magnetic resonance(NMR) and gas chromatography-mass spectrometry(GC-MS) were performed to investigate the chemical structural features of PCP-Ⅰ and its hydrolytic oligosaccharide(PCP-Ⅰ-hy-1). These results suggested that the backbone of PCP-Ⅰ was composed of galactose with α anomeric carbon and β anomeric carbon. The linking residues of galactan are(1→),(l→6) and(1→2,6).
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oligosaccharides
/
Polysaccharides
/
Carbon
/
Spectroscopy, Fourier Transform Infrared
/
Influenza A Virus, H1N1 Subtype
/
Poria
/
Galactose
/
Adjuvants, Vaccine
/
Hydrolysis
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2023
Type:
Article
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