The transcription factor ZEB1 promotes an aggressive phenotype in prostate cancer cell lines / 亚洲男科学杂志(英文版)
Asian Journal of Andrology
;
(6): 294-299, 2018.
Article
in English
| WPRIM
| ID: wpr-1009562
ABSTRACT
It has been reported that one of the factors that promotes tumoral progression is the abnormal activation of the epithelial-mesenchymal transition program. This process is associated with tumoral cells acquiring invasive and malignant properties and has the transcription factor zinc finger E-box-binding homeobox 1 (ZEB1) as one of its main activators. However, the role of ZEB1 in promoting malignancy in prostate cancer (PCa) is still unclear. Here, we report that ZEB1 expression correlates with Gleason score in PCa samples and that expression of ZEB1 regulates epithelial-mesenchymal transition and malignant characteristics in PCa cell lines. The results showed that ZEB1 expression is higher in samples of higher malignancy and that overexpression of ZEB1 was able to induce epithelial-mesenchymal transition by upregulating the mesenchymal marker Vimentin and downregulating the epithelial marker E-Cadherin. On the contrary, ZEB1 silencing repressed Vimentin expression and upregulated E-Cadherin. ZEB1 expression conferred enhanced motility and invasiveness and a higher colony formation capacity to 22Rv1 cells whereas DU145 cells with ZEB1 silencing showed a decrease in those same properties. The results showed that ZEB1 could be a key promoter of tumoral progression toward advanced stages of PCa.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Prostatic Neoplasms
/
Vimentin
/
Cadherins
/
Gene Expression Regulation, Neoplastic
/
Cell Movement
/
Gene Silencing
/
Cell Line, Tumor
/
Epithelial-Mesenchymal Transition
/
Neoplasm Grading
/
Zinc Finger E-box-Binding Homeobox 1
Limits:
Humans
/
Male
Language:
English
Journal:
Asian Journal of Andrology
Year:
2018
Type:
Article
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