Preliminary Study on the Relationship between the Developmental Stage of Multiple Myeloma Disease and the Results of Bone Marrow Whole Exome Sequencing / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 1750-1756, 2023.
Article
in Zh
| WPRIM
| ID: wpr-1010033
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To investigate the genetic results of whole exome sequencing of bone marrow from new onset multiple myeloma (MM) patients to analyze the process of genetic clonal evolution in MM patients.@*METHODS@#Genomic DNA was extracted from bone marrow samples of 15 MM patients and the whole exomes sequencing was performed using next generation sequencing technology. Using own buccal cells as germline controls, combinated with clinical information, the mutation profile of genes from high-risk asymptomatic myeloma to symptomatic myeloma were analyzed, and genes that may be associated with the efficacy and side effects of bortezomib were screened.@*RESULTS@#Except for two patients in whom no peripheral neuropathy was observed after a short treatment period, other patients peripheral neuropathy developed of various degrees during treatment with bortezomib containing chemotherapy, and the vast majority of patients achieved remission after receiving this bortezomib-related chemotherapy regimen. All patients had comparable levels of the inherited mutations number, but the somatic mutations was correlated with disease evolution.@*CONCLUSION@#different gene "mutational spectra" exist in myeloma patients at different stages and are associated with progression through all stages of the disease.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Bone Marrow
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Antineoplastic Combined Chemotherapy Protocols
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Bortezomib
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Exome Sequencing
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Mouth Mucosa
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Multiple Myeloma
Limits:
Humans
Language:
Zh
Journal:
Journal of Experimental Hematology
Year:
2023
Type:
Article