Ureaplasma urealyticum-derived lipid-associated membrane proteins introduce IL-6, IL-8, and TNF-α cytokines into human amniotic epithelial cells via Toll-like receptor 2 / 浙江大学学报(英文版)(B辑:生物医学和生物技术)
Journal of Zhejiang University. Science. B
;
(12): 654-661, 2018.
Article
in English
| WPRIM
| ID: wpr-1010402
ABSTRACT
OBJECTIVE@#The purpose of this study was to determine the role of Ureaplasma urealyticum-derived lipid-associated membrane proteins (LAMPs) in the host innate immune system, specifically their effect on Toll-like receptors (TLRs).@*METHODS@#LAMPs were derived from U. urealyticum strains, and human amniotic epithelial cells (HAECs) were isolated from healthy full-term placentas. Cytokine concentrations were determined by enzyme-linked immunosorbent assay (ELISA) and TLR2 mRNA by real-time PCR. Expression of TLR2 was confirmed by Western blotting and immunohistochemistry.@*RESULTS@#LAMPs induced HAECs to produce inflammatory cytokines interleukin (IL)-6, IL-8, and tumor necrosis factor (TNF)-α. Cytokine production was reduced after blocking TLR2 using TLR2 inhibitor (anti-hTLR2-IgA).@*CONCLUSIONS@#LAMPs isolated from U. urealyticum induced TLR2-dependent up-regulation of inflammatory genes and cytokines in HAECs.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Placenta
/
Up-Regulation
/
Lipopolysaccharides
/
Cytokines
/
Interleukin-8
/
Interleukin-6
/
Tumor Necrosis Factor-alpha
/
Ureaplasma urealyticum
/
Dose-Response Relationship, Drug
/
Epithelial Cells
Limits:
Female
/
Humans
/
Pregnancy
Language:
English
Journal:
Journal of Zhejiang University. Science. B
Year:
2018
Type:
Article
Similar
MEDLINE
...
LILACS
LIS