miRNA-128-3p inhibits malignant behavior of glioma cells by downregulating KLHDC8A expression / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1447-1459, 2023.
Article
in Chinese
| WPRIM
| ID: wpr-1010600
ABSTRACT
OBJECTIVE@#To determine whether miRNA-128-3p regulates malignant biological behavior of glioma cells by targeting KLHDC8A.@*METHODS@#Dual-luciferase reporter assays, qRT-PCR and Western blotting were used to verify the targeting of miRNA-128-3p to KLHDC8A. Edu assay, flow cytometry, Transwell assay and would healing assay were used to determine the effects of changes in miRNA-128-3p and KLHDC8A expression levels on malignant behavior of glioma cells. Rescue experiment was carried out to verify that miRNA-128-3p regulated glioma cell proliferation, apoptosis, invasion and migration by targeting KLHDC8A.@*RESULTS@#The expression level of KLHDC8A was significantly increased in high-grade glioma tissue and was closely related to a poor survival outcome of the patients. Overexpression of KLHDC8A promoted glioma cell proliferation, migration and invasion, and miRNA-128-3p overexpression inhibited proliferative and metastatic capacities of glioma cells. Mechanistically, KLHDC8A expression was directly modulated by miRNA-128-3p, which, by targeting KLHDC8A, inhibited malignant behavior of glioma cells.@*CONCLUSION@#Upregulation of miRNA-128-3p inhibits uncontrolled growth of glioma cells by negatively regulating KLHDC8A expression and its downstream effectors, suggesting that the miRNA-128-3p-KLHDC8A axis may serve as a potential prognostic indicator and a therapeutic target for developing new strategies for glioma treatment.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Gene Expression Regulation, Neoplastic
/
Up-Regulation
/
Cell Movement
/
Apoptosis
/
MicroRNAs
/
Cell Line, Tumor
/
Cell Proliferation
/
Glioma
Limits:
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2023
Type:
Article
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