Single-Cell Mapping of Brain Myeloid Cell Subsets Reveals Key Transcriptomic Changes Favoring Neuroplasticity after Ischemic Stroke / 神经科学通报·英文版
Neuroscience Bulletin
; (6): 65-78, 2024.
Article
in En
| WPRIM
| ID: wpr-1010670
Responsible library:
WPRO
ABSTRACT
Interactions between brain-resident and peripheral infiltrated immune cells are thought to contribute to neuroplasticity after cerebral ischemia. However, conventional bulk sequencing makes it challenging to depict this complex immune network. Using single-cell RNA sequencing, we mapped compositional and transcriptional features of peri-infarct immune cells. Microglia were the predominant cell type in the peri-infarct region, displaying a more diverse activation pattern than the typical pro- and anti-inflammatory state, with axon tract-associated microglia (ATMs) being associated with neuronal regeneration. Trajectory inference suggested that infiltrated monocyte-derived macrophages (MDMs) exhibited a gradual fate trajectory transition to activated MDMs. Inter-cellular crosstalk between MDMs and microglia orchestrated anti-inflammatory and repair-promoting microglia phenotypes and promoted post-stroke neurogenesis, with SOX2 and related Akt/CREB signaling as the underlying mechanisms. This description of the brain's immune landscape and its relationship with neurogenesis provides new insight into promoting neural repair by regulating neuroinflammatory responses.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Brain
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Brain Ischemia
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Microglia
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Gene Expression Profiling
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Ischemic Stroke
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Infarction
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Macrophages
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Anti-Inflammatory Agents
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Neuronal Plasticity
Limits:
Humans
Language:
En
Journal:
Neuroscience Bulletin
Year:
2024
Type:
Article