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Single-Cell Mapping of Brain Myeloid Cell Subsets Reveals Key Transcriptomic Changes Favoring Neuroplasticity after Ischemic Stroke / 神经科学通报·英文版
Neuroscience Bulletin ; (6): 65-78, 2024.
Article in En | WPRIM | ID: wpr-1010670
Responsible library: WPRO
ABSTRACT
Interactions between brain-resident and peripheral infiltrated immune cells are thought to contribute to neuroplasticity after cerebral ischemia. However, conventional bulk sequencing makes it challenging to depict this complex immune network. Using single-cell RNA sequencing, we mapped compositional and transcriptional features of peri-infarct immune cells. Microglia were the predominant cell type in the peri-infarct region, displaying a more diverse activation pattern than the typical pro- and anti-inflammatory state, with axon tract-associated microglia (ATMs) being associated with neuronal regeneration. Trajectory inference suggested that infiltrated monocyte-derived macrophages (MDMs) exhibited a gradual fate trajectory transition to activated MDMs. Inter-cellular crosstalk between MDMs and microglia orchestrated anti-inflammatory and repair-promoting microglia phenotypes and promoted post-stroke neurogenesis, with SOX2 and related Akt/CREB signaling as the underlying mechanisms. This description of the brain's immune landscape and its relationship with neurogenesis provides new insight into promoting neural repair by regulating neuroinflammatory responses.
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Full text: 1 Index: WPRIM Main subject: Brain / Brain Ischemia / Microglia / Gene Expression Profiling / Ischemic Stroke / Infarction / Macrophages / Anti-Inflammatory Agents / Neuronal Plasticity Limits: Humans Language: En Journal: Neuroscience Bulletin Year: 2024 Type: Article
Full text: 1 Index: WPRIM Main subject: Brain / Brain Ischemia / Microglia / Gene Expression Profiling / Ischemic Stroke / Infarction / Macrophages / Anti-Inflammatory Agents / Neuronal Plasticity Limits: Humans Language: En Journal: Neuroscience Bulletin Year: 2024 Type: Article