The renal protective effect of Mongolian medicine rhubarb-3 decoction on rats with chronic renal failure based on TGF-(i/Smad signaling pathway / 中国药理学通报

ABSTRACT

Aim To study the protective effect of Mon¬golian medicine rhubarb-3 decoction on renal function of CRF model rats and explore its mechanism.Meth¬ods SD rats were randomly divided into normal group and model group.The model group was established by adenine gavage.After successful modeling, they were randomly divided into model, positive, rhubarb-3 de¬coction low, medium and high dose groups, which were administered at intervals for 12 weeks.The bio¬chemical detection of CSF, BUN and Scr was per¬formed.The pathological changes of renal tissues were observed by HE and Masson staining.The expression of PCNA and a-SMA in renal tissues was detected by immunohistochemistry.The mRNA expressions of CK18, Vimentin, TGF-pi and FN in kidney tissues were detected by RT-PCR.The expressions of a-SMA, E-cadherin, PCNA, Smad2, Smad3 protein in kidney tissue were detected by Western blot.TGF-pi induced abnormal proliferation of I IMC and interstitial transfor¬ mation of HK-2, and at the same time, it was treated with serum containing rhubarb-3 decoction.The prolif¬eration of HMC was detected by CCK-8.Interstitial transformation of HK-2 was detected by RT-PCR.Re- suits Compared with the model group, BUN and Scr in CRF rats decreased, and the expression of fibrosis- related proteins and genes in renal tissue decreased af¬ter rhubarb-3 decoction treatment.The serum contai¬ning rhubarb-3 decoction significantly inhibited HMC proliferation and HK-2 interstitial transformation.Con¬clusions Rhubarb-3 Decoction can improve renal function in CRF model rats, and its mechanism may be related to inhibiting HMC proliferation and HK-2 inter¬stitial transformation and probably regulating TGF-f}/ Smad signaling pathway.