Protective effect of fluoxetine against hypoxia induced injury on PC12 cells / 中国药理学通报

ABSTRACT

Aim To study the protective effect of fluoxetine against hypoxia induced injury on PC12 cells. Methods PC12 cells were randomly divided into control group, hypoxia group, and fluoxetine hydrochloride group. The last two groups were put into a hypoxic culture chamber for 18 hours, the cell state was observed under inverted microscope, and cell viability was detected using CCK-8 assay. Reactive oxygen species (ROS) level was evaluated by DCFH-DA. Lactate dehydrogenase (LDH), superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA) in cell culture supernatant were evaluated by enzyme labeling method. The expression levels of Bcl-2, Bax and caspase-3 were determined by Western blot. Results Compared with normal group, hypoxia caused obvious damage to PC12 cells. Fluoxetine hydrochloride at 10