Role of IL-22/IL-22BP axis in hepatic ischemia-reperfusion injury / 中国药理学通报

ABSTRACT

Aim To investigate the role of 1L-22 / IL- 22BP axis in hepatic ischeniia-reperfusion injury and its potential mechanism.Methods Short, medium, and long-term liver ischemia-reperfusion injury (IKI) and IRI + IL-22 mouse models were established, then the scrum IL-22 concentration, IL-22BP mRNA, IL- 22R a 1 mRNA and STAT3 pathway related protein expression in liver were detected to study the role and mechanism of IL-22 and IL-22 BP in liver IRI.Results Compared with short-term ischemia-reperfusion injury group, mice in middle and long-term ischemia- reperfusion injury groups showed more severe liver injury.The concentration of serum IL-22 significantly increased but the activation of STAT3 pathway was significantly inhibited in long-term ischernia-reperfusion group.The ratio of IL-22BP / IL-22R a 1 niRNA increased significantly with the prolongation of ischemia time.Liver injury was significantly alleviated and the activation of STAT3 pathway was markedly up-regulated after the administration of exogenous recombinant 1L-22 in mice with long-term ischemia-reperfusion injury.Conclusions IL-22 can protect liver from ischemia-reperfusion injur)'; however, IL-22BP / IL-22R a 1 mRNA ratio is a negative indicator of liver injury, and the mechanism may be related to the regulation of STAT3 pathway activation by IL-22 / il-22bp axis dur-ing liver IRI.